My Account      Contact Us      About Us     
Custom Antibody and Recombinant Protein Experts

VAPR Custom Antibodies and Proteins

The Vanderbilt Antibody and Protein Resource (VAPR) partners both with scientists locally and from across the country to design and develop reagents for research, diagnostics, and large-molecule therapeutics.

Custom Monoclonal Antibody Services

  • Generate Novel Monoclonal Antibodies. We have experience in generating reasearch antibodies, therapeutic/neutralizing antibodies, phospho-site specific antibodies, splice/cleavage site specific antibodies, and many others.
  • Large-Scale purification of monoclonal antibodies from existing hybridomas
  • Antibody labeling
  • Antigen generation and purification
  • Hybridoma optimization

Polyclonal Antibody Services

  • Polyclonal antibody production
  • Affinity purification of polyclonal antibodies
  • Antigen generation and purification

Recombinant Antibody / Antibody Engineering Services

  • Isolation and sequencing of antibody variable (VH and VL) domains from hybridomas
  • Small and large-scale antibody productions/purifications from cloned antibodies
  • Antibody humanization (chimera generation)
  • Single-chain (scFv) generation
  • Antibody Optimization

Recombinant Protein Services

  • Recombinant protein expression, optimization, and purification
    • Codon optimization of target for maximum expression
    • ​Bacterial based expression
    • Mammalian cell based expression (transient and stable)
    • Bacculovirus based expression
  • Kinetic and Quantitative Analyses of Protein:Protein and Protein:Small Molecule Interactions

Assay and Labeling Services

  • Sandwich and Direct ELISA assay development
    • ​Initial antibody pairing and standard validation
    • Assay qualification
    • ELISA testing service with custom or commercial assays
  • Services to perform most antibody-based assays 
  • Protein and Antibody labeling/conjugation
    • Any scale
    • Carriers, Resins, Magnetic beads, etc
    • Fluorophores, CyTOF labels, etc

General Purpose antibodies and Enzymes

  • VAPR offers extremely high-quality purified antibodies and molecular biology enzymes to Vanderbilt Investigators.  These are fully quality tested and 100% guaranteed.  To see the full list of available reagents go to the repositories web page.

Click here or on the Services link on the left side of the screen

to see a full menu of available services